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An in vitro assessment of the potential toxicity of Cadmium Selenide nanoparticles

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dc.contributor.advisor Shonhai, A.
dc.contributor.advisor Opoku, A.R.
dc.contributor.advisor Revaprasadu, N.
dc.contributor.author Dunpall, Rekha
dc.date.accessioned 2012-06-05T12:53:51Z
dc.date.available 2012-06-05T12:53:51Z
dc.date.issued 2012
dc.identifier.uri http://hdl.handle.net/10530/1060
dc.description Dissertation submitted to the Department of Biochemistry and Microbiology in the Faculty of Science and Agriculture, University of Zululand (South Africa) in fulfillment of the requirements for the Master (Msc) degree in Biochemistry, 2012. en_US
dc.description.abstract Nanotechnology is steadily finding its application in all aspects of the consumer industry, science and engineering. At a relative pace Cadmium Selenide (CdSe) nanoparticles are gaining increased attention for their potential use in biomedical applications such as bioimaging of tissues, disease diagnosis and biological labelling due to its unique optical and electronic properties. Exposure of these particles to humans and other biological systems raise huge concerns with regards to their safety. In this study, water soluble cysteine capped CdSe nanocrystals, were prepared through a one pot green route method. The prepared CdSe nanocrystals were characterized using Transmission electron microscopy (TEM), High resolution transmission electron microscopy (HRTEM), Ultra violet spectroscopy (UV) and Photoluminescence (PL) analysis to establish the size, shape, dispersion, aggregation state, crystalline nature and optical properties of CdSe nanoparticles. The in vitro effects of CdSe nanoparticles on DNA stability, red blood cells (RBC’s) and blood platelets were evaluated. DNA was exposed to CdSe nanoparticles and its assessment on DNA stability was confirmed by agarose gel electrophoresis and spectrophotometry. Damage to DNA structure was observed at 200 μg/ml of CdSe. In vitro assays carried out on RBC damage included reducing power and chelating activity of iron. The results showed that the CdSe nanocrystals exhibited high reducing power and sufficient chelating activity, which would be able to impair the function of haemoglobin. CdSe nanoparticles promoted platelet aggregation in a dose dependent manner. Based on the findings of this study the biosafety of CdSe nanoparticles is not guaranteed and further studies need to be conducted to ascertain the safety of CdSe nanoparticles for possible use in biological systems. en_US
dc.description.sponsorship National Research Foundation (NRF) of South Africa, & the Department of Science & Technology of South Africa. en_US
dc.language.iso en en_US
dc.subject Nanoparticle - Cadmium Selenide (CdSe) en_US
dc.subject Disease diagnosis and drug delivery en_US
dc.title An in vitro assessment of the potential toxicity of Cadmium Selenide nanoparticles en_US
dc.type Thesis en_US

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